Substrate Database


Instrument and accessories

Agilent 6890 gas chromatograph and 5973 mass spectrometer
Agilent ALS autosampler G2614A

GC conditions

Column: HP-1 or equivalent
Length: 25 meters
Inner diameter: 0.20 mm
Film thickness: 0.50 microns
Carrier gas: Helium (ultra high purity)
Constant flow
Flow rate: 0.80 ml/min.
Linear velocity: approximately 36 cm/sec
Split ratio: 50/1
Injection volume: 1 microliter
Pre injection washes of sample: 2
Pumps: 2
Post injection wash of pentane: 10
Post injection wash of pentane: 10
Fast plunger
Merlin microseal
Injection temperature: 250 degrees C
Initial hold time: 3 min.
Rate: 10 degrees C/min.
Final temperature: 280 degrees C
Final hold time: 4 min.
Total run time: 30 min.


MS conditions

Transfer line temperature: 280 degrees C
Acquisition mode: scan
Scan range: 30 – 350
Scan rate: 2-3 scans/sec; equivalent to 6 -10 scans per peak
Solvent delay: 2 min.

As of November 2010: no solvent delay, however the detector is off between 1.54 and 2.00 minutes