Instrument Parameters     1. Instrument and accessories Agilent (Hewlett-Packard) 6890 gas chromatograph and 5973 mass spectrometer Agilent ALS autosampler G2614A  2. GC conditions: Column: HP-1 or equivalent Length: 25 meters Inner diameter: 0.20mm Film thickness: 0.5 microns Carrier gas: Helium (high purity grade) Constant flow Flow rate: 0.8 ml/min. Linear velocity: approximately 36 cm/sec Split ratio: 50/1 Injection volume: 1 microliter Pre injection washes of sample: 2 Pumps: 2 Post injection wash of solvent A: 10 Post injection wash of solvent B: 10 Fast plunger Merlin microseal Injection temperature: 250 degrees C Initial temperature: 50 degrees C Initial hold time: 3 min Rate: 10 degrees C/min Final temperature: 280 degrees C Final hold time: 4 min Total run time: 30 min 3. MS conditions:                Transfer line temperature: 280 degrees C Acquisition mode: scan Scan range: 30-350 Scan rate: 2-3 scans/sec; equivalent to 6-10 scans per peak Solvent delay: 2 min As of November 2010: no solvent delay, however the detector is off between 1.54 and 2.00 minutes   Copyright © 2001-2006 University of Central Florida. All Rights Reserved.